Chameleon Kit: Mix and match Chameleon 700 and Chameleon 800 to create the best protein ladder for your experimental needs. New England Biolabs offers a selection of highly pure protein standards.Chameleon 800: 6 multi-colored, pre-stained proteins.These protein standards and ladders are consistent from lot to lot and are strictly. Each protein standard or ladder is supplied in a ready-to-use format, eliminating the need to reduce, pre-mix, or add loading dyes. Chameleon 700: 7 multi-colored, pre-stained proteins Western blotting (WB) is a fundamental technique that has Comparable MW detection with competitors’ protein ladders helped scientists understand important signaling pathways, protein associations, protein expression in various cell lines and tissues, and effects of drugs or treatments on protein levels. Choose from a variety of protein ladders (molecular weight markers) for protein electrophoresis and western blotting applications.Chameleon Duo: 11 multi-colored, pre-stained bands Proteins can be detected on Western blots by staining with Ponceau S, Coomassie Blue, (except for 3.4kDa and 5kDa polypeptides) or by using Strep- Tactin conjugates.Protein Ladders Provide Reliable MW Marker Sequences Not sure which protein marker to choose? Visit How to Choose the Right Protein Ladder. The protein ladder is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce, or add. Consisting of recombinant proteins fused to an IgG binding site, these versatile molecular weight ladders bind the primary or secondary antibody used for the detection of the target protein. Thermo Scientific PageRuler Prestained Protein Ladder is a mixture of 10 blue-, orange-, and green-stained proteins (10 to 180 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. For special cases, use the Chameleon Kit to customize a two-color protein ladder that suits the needs of your experiment. Western blot protein ladders are designed for protein molecular weight estimation directly on the blot during detection. Chameleon Duo can be used to perform two-color visible and near-infrared detection. Easily visualize gel migration and protein sizeĬhameleon Pre-stained Protein Ladders are optimized for use with Bis-Tris and Tris-Glycine gels.Ĭhameleon 700 or Chameleon 800 protein ladders can be used for one-channel analysis.Ensure good size coverage with Chameleon ladders ranging from 8 kDa to 260 kDa.Estimate molecular weights of your proteins using these broad-range recombinant proteins The protein standard is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce, or add sample buffer prior to use.Under suggested conditions, BLUeye Prestained Protein Ladder resolves 12 major bands in 15 % SDS-PAGE (Trisglycine buffer) and after Western blotting to nitrocellulose membrane.Īpproximately 0.1~0.4 mg/mL of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25 ☌), 2 % SDS, 1 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).Chameleon near-infrared (NIR) pre-stained protein ladders offer multi-colored, pre-stained proteins for both visual inspection and two-color near-infrared fluorescent detection. The ladder is supplied in gel loading buffer and is ready to use. The BLUeye Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The BLUeye Prestained Protein Ladder is a three-color protein standard with 12 pre-stained proteins covering a wide range molecular weights from 10 to 245 kDa. Precision Plus Protein WesternC Standards are combination protein standards containing ten prestained, Strep-tagged recombinant proteins, enabling both fluorescent visualization on gels and colorimetric or chemiluminescent western blot detection with StrepTactin-AP or StrepTactin-HRP Conjugate. 1.5-2.5 μL per well for general Western transfering.īLUeye Prestained Protein Ladder resolves 12 major bands in 15 % SDS-PAGE (Tris-glycine buffer) and after Western blotting to nitrocellulose membrane. Use 3 μL or 5 μL per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. Custom Recombinant Antibody (rAbs) Services.Annexin V-FITC Apoptosis Detection Kits.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |